Table 1. List of antibodies and cocktail configurations used for myosin heavy chain (MHC) staining by multicolor immunofluorescence

Species	Method	Sequence	Primary antibodies	MHC reactivity	Secondary antibody
Bovine	Cocktail	1st	BA-F8	I/slow	Alexa Flour647 IgG2b (purple)
			SC-71	2a, 2x	Alexa Flour488 IgG1 (green)
			6H1	I/slow,2x	Alexa Flour405 IgM (blue)
		2nd	BF-35	I/slow, 2a	Alexa Flour594 IgG1 (red)
	Cocktail	1st	BA-F8	I/slow	Alexa Flour647 IgG2b (purple)
			SC-71	2a, 2x	Alexa Flour594 IgG1 (red)
		2nd	BF-35	I/slow, 2a	Alexa Flour488 IgG1(green)
Porcine	Cocktail	1st	BA-F8	I/slow	Alexa Flour647 IgG2b (purple)
			SC-71	I/slow, 2a, 2x	Alexa Flour488 IgG1 (red)
		2nd	BF-35	I/slow, 2a	Alexa Flour594 IgG1 (green)
			BF-F3	2b	Alexa Flour405 IgM (blue)
	Serial		BA-F8	I/slow	Alexa Flour647 IgG2b (purple)
			SC-71	2a, 2x	Alexa Flour488 IgG1 (green)
			BF-35	I/slow, 2a	Alexa Flour594 IgG1 (red)
			BF-F3	2b	Alexa Flour405 IgM (blue)