Table 3. The effect of nitroso-hemoglobin and storage period (13–16 d) on combined bacteria in the ratio (1:1) injected in minced beef

The effect of NO-Hb and storage for 13 d on the combine bacteria in the minced beef
Bacteria in the ratio (1:1) injected in fresh beef without NO-Hb
Parameters	TBC	Escherichia coli	Staphylococcus	Pseudomonas	Salmonella	LAB
Log10⁻¹ CFU/g	Un	1.03±0.02^b	1.27±0.02^a	Un	0.43±0.00^c	Un
Log10⁻² CFU/g	Un	0.12±0.00^a	OR	Un	OR	Un
Log10⁻³ CFU/g	Un	-	-	2.26±0.85^b	-	2.64±0.02^a
Log10⁻⁴ CFU/g	2.52±0.02^a	-	-	1.07±0.00^c	-	1.71±0.01^b
Bacteria in the ratio (1:1) injected in fresh beef with NO-Hb
Log10⁻¹ CFU/g	2.67±0.01^a	-	-	0.52±0.00^c	-	2.12±0.88^b
Log10⁻² CFU/g	1.67±0.00a	-	-	-	-	1.29±0.01^b
Log10⁻³ CFU/g	0.58±0.00^a	-	-	-	-	0.22±0.00^b
Log10⁻⁴ CFU/g	OR	-	-	-	-	-
The effect of NO-Hb and storage for 16 d on the combine bacteria in the minced beef
Bacteria in the ratio (1:1) injected in fresh beef without NO-Hb
Log10⁻¹ CFU/g	3.73±0.01^a	0.75±0.00^e	0.94±0.00^d	1.57±0.00^c	0.22±0.00^f	2.54±0.33^b
Log10⁻² CFU/g	1.64±0.02^a	-	OR	0.75±0.00^c	-	1.47±0.01^b
Log10⁻³ CFU/g	0.88±0.00^a	-	-	OR	-	0.75±0.00^a
Log10⁻⁴ CFU/g	OR	-	-	-	-	OR
Bacteria in the ratio (1:1) injected in fresh beef with NO-Hb
Log10⁻¹ CFU/g	0.92±0.00^a	-	-	-	-	0.80±0.00^b
Log10⁻² CFU/g	OR	-	-	-	-	OR
Log10⁻³ CFU/g	-	-	-	-	-	-
Log10⁻⁴ CFU/g	-	-	-	-	-	-

Values are given as means±SD from triplicate determinations.
The valid range we used in our study is ranging from 25–250 (Log₁₀ CFU).
Different letters in the same row indicate significant differences (p<0.05).
TBC, total bacterial count, Escherichia coli, (E. coli O157:H7, E. coli (ETEC), E. coli (EHEC), Staphylococcus, (S. aureus, S. argenteus, S. simiae), Pseudomonas, (P. lundensis, P. mudicolens, P. aeruginosa), Salmonella, (S. Typhimurium, S. Javiana, S. Enteritidis), LAB, (S. thermophilus, L. bulgaricus, L. casei, and M. enterococcus); Un, uncountable=too numerous to count (TNTC): more than 300 colonies; -, not detected; OR, out of range. Log10⁻¹ CFU/g, first dilution; Log10⁻² CFU/g, second dilution; Log10⁻³ CFU/g, third dilution; Log10⁻⁴ CFU/g, fourth dilution.