Table 1. The effect of NO-Hb on individual bacterial counts in the ratio (1:1) injected in fresh beef

Prior inoculation Log10 CFU /g−1
Parameters TBC Escherichia coli Staphylococcus Pseudomonas Salmonella Lactic acid bacteria
E. A E. B E. C St. A St. B St. C P. A P. B P. C S. A S. B S. C L. bulgaricus L. casei M. enterococcus S. thermophilus
 Log10−1 CFU/g 0.58±0.01a 0.12±0.00c OR 0.02±0.00d - - - OR - - - - - - 0.25±0.00b - -
Bacteria in the ratio (1:1) injected in fresh beef without NO-Hb
 Log10−1 CFU/g Un Un Un Un Un Un Un Un Un Un Un Un Un Un 2.14±0.42a Un 1.58±0.02b
 Log10−2 CFU/g Un Un 1.42±0.00f 2.05±0.01e 2.95±1.35a 2.06±0.04e 1.14±0.00g Un 2.06±0.02e 2.84±0.02b Un 2.62±0.02d 2.06±0.02e 2.78±0.02c 1.05±0.04g 2.71±0.40c 1.17±0.04g
 Log10−3 CFU/g Un 2.62±0.36c 0.42±0.00h 1.42±0.00g 1.73±0.02d 0.12±0.00i OR 2.70±0.37b 1.24±0.02g 0.58±0.00h 2.82±0.01a 1.52±0.01e 0.58±0.00h 1.85±0.04e OR 1.64±0.08f 0.79±0.00h
 Log10−4 CFU/g Un 2.06±0.12a - OR 1.10±0.02c OR - 1.62±0.02b OR OR 1.8±0.55b OR - 0.88±0.00d - 0.56±0.00d -
Bacteria in the ratio (1:1) injected in fresh beef with NO-Hb
 Log10−1 CFU/g Un 2.56±0.02b 2.16±0.04c 2.11±0.01c 2.07±0.04c 2.53±0.03b 2.08±0.02c 1.87±0.02d 1.76±0.04d 1.86±0.02d 2.86±0.04a 2.16±0.02c 2.21±0.04c 2.17±0.04c 1.56±0.00e 2.52±0.02b 0.56±0.00f
 Log10−2 CFU/g Un 1.56±0.04c 1.82±0.00a 1.56±0.02c 1.58±0.39c 0.86±0.00e 0.56±0.00f 0.64±0.00f 1.52±0.00c 0.58±0.00f 1.20±0.04d 1.24±0.02d 1.20±0.02d 1.61±0.04b 0.85±0.02e 1.22±0.04d -
 Log10−3 CFU/g 2.02±0.02a 0.78±0.00b 0.57±0.04c 0.98±0.00b 0.56±0.00c OR OR OR 0.24±0.02d - 0.25±0.01d 0.52±0.01c 0.56±0.01c 0.87±0.04b - 0.88±0.02b -
 Log10−4 CFU/g 0.56±0.00a - - OR OR - - - - - - - OR - - OR -
Values are given as means±SD from triplicate determinations.
The valid range we used in our study is ranging from 25–250 (Log10 CFU).
Different letters in the same row indicate significant differences (p<0.05).
TBC, total bacterial count (E. coli, Staphylococcus, Pseudomonas, Salmonella, lactic acid bacteria); E. A, E. coli O157:H7; E.B, E. coli (ETEC); E. C, E. coli (EHEC); St. A, S. aureus; St. B, S. argenteus; St. C, S. simiae; P. A, P. lundensis; P. B, P. mudicolens; P. C, P. aeruginosa; S. A, S. Typhimurium; S. B, S. Javiana; S. C, S. Enteritidis; Un, uncountable=too numerous to count (TNTC): more than 300 colonies; -, not detected; OR, out of range. Log10−1 CFU/g, first dilution; Log10−2 CFU/g, second dilution; Log10−3 CFU/g, third dilution; Log10−4 CFU/g, fourth dilution.