Table 1. Summary of tested buffers according to each condition for optimization

Buffer	Condition	Component
Lysis buffer	Native	A: 20 mM Tris, 10 mM NaCl (pH 8.0)B: 50 mM NaH2PO4, 300 mM NaCl (pH 8.0)
	Denatured	50 mM NaH2PO4, 300 mM NaCl, 8M urea (pH 8.0)
Wash buffer	Native	A: 20 mM Tris, 10 mM NaCl, 20 mM imidazole (pH 8.0)B: 50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole (pH 8.0)
	Denatured	50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, 8M urea (pH 8.0)
Elution buffer	Native	A: 20 mM Tris, 10 mM NaCl, 250 mM imidazole (pH 8.0)B: 50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole (pH 8.0)
	Denatured	50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, 8M urea (pH 8.0)