Strain |
Lactobacillus plantarum LP27 | Source of plasmid pLP27 | This study |
L. plantarum SK156 | Transformation host, erythromycin resistance negative | This study |
Escherichia coli DH5α | Transformation host for cloning | Takara Bio Inc. (Japan) |
Plasmid |
pUC19 | pBR322 replication origin, lacZ, Ampr, multicloning site | Takara Bio Inc. (Japan) |
pNZ8008 | Source of β-glucuronidase gene (gusA) | Mo Bio Laboratories (USA) |
pLP27 | Cryptic plasmid from L. plantarum LP27 | This study |
pULP1 | pUC19 with pLP27 fragment | This study |
pULP2 | pULP1 with erythromycin resistance gene (Emr) | This study |
pULP3-xxx1) | pULP2 with promoter xxx and gusA gene | This study |