Table 1. Bacterial strains and plasmids used in this study

Strain or plasmid Characteristics Source or reference
Strain
Lactobacillus plantarum LP27 Source of plasmid pLP27 This study
L. plantarum SK156 Transformation host, erythromycin resistance negative This study
Escherichia coli DH5α Transformation host for cloning Takara Bio Inc. (Japan)
Plasmid
 pUC19 pBR322 replication origin, lacZ, Ampr, multicloning site Takara Bio Inc. (Japan)
 pNZ8008 Source of β-glucuronidase gene (gusA) Mo Bio Laboratories (USA)
 pLP27 Cryptic plasmid from L. plantarum LP27 This study
 pULP1 pUC19 with pLP27 fragment This study
 pULP2 pULP1 with erythromycin resistance gene (Emr) This study
 pULP3-xxx1) pULP2 with promoter xxx and gusA gene This study
xxx: promoter EIIDMan, LDH, HPrK, or DDL.