Table 3 Effects of pH, temperature and various enzymes on the anti-listerial substance obtained from Leuconostoc lactis SD501

Treatment Residual activity1)
None (Control) +
Enzyme2)
        α-Amylase +
        Lipase +
        Proteinase-K -
        Trypsin +
        α-Chymotrypsin -
        Pepsin +
Heat:
        25℃ +
        50℃ +
        60℃ +
        80℃ +
        100℃ +
        121℃ +
pH:
        2 ++
        3 +
        4 +
        5 +
        6 +
        7 +
        8 +
        9 +
        10 +
1) Residual activity compared with antibacterial activity prior to treatment. Activity is expressed based on the diameter of the inhibition zone around the well. Degrees of inhibition around the well: −, no inhibition; +, up to 20 mm; ++, up to 25 mm.
2) The enzyme concentration used was 5 mg/mL.