Table 2 Purification of the SD501 bacteriocin by ammonium sulfate (AS) precipitation, Amberlite XAD-16N and hydrophobic C₁₈ column

Fraction	Volume(mL)	Total protein(mg)	Total activity(AU)	Specific activity(AU/mg)	Purification(fold)	Yield(%)
Culture supernatant	4,000	480	2,800,000	5,833	1	100
AS precipitation	8	84.8	890,400	10,500	1.8	32
Amberlite XAD-16N	2	12.6	360,120	28,581	4.9	13
C18 column	1	0.24	11,339	47,247	8.1	0.4

AU, activity units.