Table 3 Capture efficiency (CE) of immunomagnetic beads (IMB) against S. Typhimurium in ham, beef, egg, and cheese samples
Food samples | Ham | Beef | Egg | Cheese (Ricotta) |
Inoculumconcentration(CFU/g) | CE (%) of IMB method (CFU/mL) | CE (%) of conventional method (CFU/mL) | CE (%) of IMB method (CFU/mL) | CE (%) of conventional method (CFU/mL) | CE (%) of IMB method (CFU/mL) | CE (%) of conventional method (CFU/mL) | CE (%) of IMB method (CFU/mL) | CE (%) of conventional method (CFU/mL) |
101 | 9.1±1.3cd*(7.7×100) | 0.0±0.0e | 8.7±1.8bc*(5.7×100) | 0.0±0.0e | 7.5±1.1cd*(4.7×100) | 0.0±0.0f | 8.4±0.0bc*(8.3×100) | 0.0±0.0e |
102 | 19.8±1.5b*(9.3×101) | 2.3±0.6e(1.1×101) | 13.8±1.7a*(4.8×101) | 0.7±0.2e(2.3×100) | 10.7±1.2ab*(2.9×101) | 2.0±0.2e(5.0×100) | 10.8±0.8ab*(9.9×101) | 1.3±0.2e(1.2×101) |
103 | 26.0±0.4a*(1.0×103) | 1.5±0.1e(5.8×102) | 12.7±0.3a*(4.6×102) | 0.7±0.1e(2.1×101) | 11.3±1.3*(4.3×102) | 0.3±0.1ef(1.1×101) | 11.5±1.7a*(1.1×103) | 1.0±0.1e(9.0×101) |
104 | 16.5±0.5b*(9.9×103) | 1.1±0.1e(6.3×102) | 10.2±0.9b*(5.4×103) | 0.8±0.0e(4.7×102) | 9.1±1.7cb*(2.4×103) | 0.5±0.1ef(1.3×102) | 12.1±0.2a*(9.9×103) | 0.6±0.1e(4.6×102) |
105 | 12.4±0.7c*(4.7×104) | 0.8±0.1cd(3.2×103) | 7.2±0.6dc*(3.1×104) | 1.2±0.1e(6.1×103) | 7.5±0.2cd*(3.6×104) | 0.3±0.1ef(1.4×103) | 6.5±0.4cd*(5.6×104) | 0.4±0.1e(3.8×103) |
106 | 7.0±1.0d*(2.9×105) | 0.7±0.1e(2.7×104) | 5.8±0.4d*(2.3×105) | 0.9±0.1e(4.7×104) | 6.0±1.2d*(2.1×105) | 0.4±0.1ef(1.4×104) | 4.6±1.3d*(3.7×105) | 0.1±0.1e(1.2×104) |
Means±SE with different superscript letters within the same column differ significantly at p<0.05.
* Significant difference (p<0.05) in CE between IMB-based method and conventional method at a given inoculum concentration in a given food sample.