| Component (%) | MRS medium | Optimum medium |
|---|---|---|
| Soy peptone | - | 0.231 |
| Proteose peptone | 1.0 | - |
| Glucose | 2.0 | 1.232 |
| Yeast extract | 0.5 | 1.97 |
| Magnesium sulfate | 0.01 | 0.08 |
| Ammonium citrate | 0.2 | - |
| Beef extract | 1.0 | - |
| Manganese sulfate | 0.005 | - |
| Potassium phosphate dibasic | 0.2 | - |
| Sodium acetate | 0.5 | - |
| Tween 80 | 0.1 | - |
| Viable cells (Log CFU/mL) | 9.51 | 9.15 |