Table 1 Treatment combinations and responses

No.	Block	Peptone	Glucose	Yeast extract	Magnesium sulfate	OD	Log (CFU)
		(g/L) [×1]	(g/L) [×2]	(g/L) [×3]	(g/L) [×4]
1	0	1 [–1]	7 [–1]	5 [–1]	0.5 [–1]	1.532	8.72098574
2	0	3 [ 1]	7 [–1]	5 [–1]	0.5 [–1]	1.328	8.98944982
3	0	1 [–1]	21 [ 1]	5 [–1]	0.5 [–1]	1.171	8.6127839
4	0	3 [ 1]	21 [ 1]	5 [–1]	0.5 [–1]	1.33	8.61278386
5	0	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.577	8.979093
6	0	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.589	8.960471
7	1	1 [–1]	7 [–1]	15 [ 1]	0.5 [–1]	1.741	8.90471554
8	1	3 [ 1]	7 [–1]	15 [ 1]	0.5 [–1]	1.714	8.95760729
9	1	1 [–1]	21 [ 1]	15 [ 1]	0.5 [–1]	1.666	8.9444827
10	1	3 [ 1]	21 [ 1]	15 [ 1]	0.5 [–1]	1.695	8.89762709
11	1	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.524	8.829947
12	1	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.548	8.85309
13	2	1 [–1]	7 [–1]	5 [–1]	1.5 [ 1]	1.166	8.63346845
14	2	3 [ 1]	7 [–1]	5 [–1]	1.5 [ 1]	1.293	8.63346846
15	2	1 [–1]	21 [ 1]	5 [–1]	1.5 [ 1]	1.14	8.6263404
16	2	3 [ 1]	21 [ 1]	5 [–1]	1.5 [ 1]	1.322	8.69722934
17	2	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.554	8.878522
18	2	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.566	8.860937
19	3	1 [–1]	7 [–1]	15 [ 1]	1.5 [ 1]	1.723	8.85491302
20	3	3 [ 1]	7 [–1]	15 [ 1]	1.5 [ 1]	1.763	8.99694925
21	3	1 [–1]	21 [ 1]	15 [ 1]	1.5 [ 1]	1.678	8.8481891
22	3	3 [ 1]	21 [ 1]	15 [ 1]	1.5 [ 1]	1.684	8.88817949
23	3	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.457	8.90309
24	3	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.506	8.742725
25	4	0 [–2]	14 [ 0]	10 [ 0]	1 [ 0]	1.101	8.42488163
26	4	4 [ 2]	14 [ 0]	10 [ 0]	1 [ 0]	1.531	8.53529412
27	4	2 [ 0]	0 [–2]	10 [ 0]	1 [ 0]	0.701	8.3283796
28	4	2 [ 0]	28 [ 2]	10 [ 0]	1 [ 0]	1.433	8.64443859
29	4	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.423	8.740363
30	4	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.427	8.799341
31	5	2 [ 0]	14 [ 0]	0 [–2]	1 [ 0]	0.409	8.23299611
32	5	2 [ 0]	14 [ 0]	20 [ 2]	1 [ 0]	1.905	9.14921911
33	5	2 [ 0]	14 [ 0]	10 [ 0]	0 [–2]	1.409	8.8802418
34	5	2 [ 0]	14 [ 0]	10 [ 0]	2 [ 2]	1.722	9.071882
35	5	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.591	8.888179
36	5	2 [ 0]	14 [ 0]	10 [ 0]	1 [ 0]	1.596	8.857332