Table 2. HPLC operating conditions for free amino acids analysis in cheese

Items		Conditions
Mobile phase		A: aqueous buffer, Waters AcccQ-Tag Eluent A
		B: 60% acetonitrile
Column		Nova-PAKTMC18, 4 μm
Column temperature		37°C
Flow rate		1 mL/min
Detector		Excitation wavelength: 250 nm
		Emission wavelength: 395 nm
Time (min)	Flow rate	A (%)	B (%)
Initial	1.0	100	0
0.5	1.0	98	2
15	1.0	93	7
19	1.0	90	10
32	1.0	67	33
33	1.0	67	33
34	1.0	0	100
37	1.0	0	100
38	1.0	100	0
64	1.0	100	0
65	1.0	0	100
100	1.0	0	100