Table 3. Free amino acids contents of top round and shank muscles with three factors (muscle type, aging method, and aging time)

mg/kg Muscle type Aging methods Aging time (d) p-value

Top round Shank p-value Dry Wet p-value 20 40 p-value MT*AM1) AM*AT2)
Asp 225.67 793.67 <0.001 527.49 491.85 <0.001 263.54 755.80 <0.001 <0.001 <0.001
Glu 2462.12 4247.87 <0.001 3574.64 3135.34 <0.001 2938.23 3771.76 <0.001 <0.001 <0.001
Asn 410.80 175.44 <0.001 112.73 473.50 <0.001 249.74 336.49 <0.001 <0.001 <0.001
Ser 1109.44 1020.65 0.014 1050.42 1079.67 0.332 1132.90 997.19 0.001 0.005 0.011
Gln 1629.52 771.27 <0.001 330.84 2069.94 <0.001 576.62 1824.17 <0.001 0.549 <0.001
His 671.32 1017.81 <0.001 1023.63 665.50 <0.001 791.75 897.38 0.021 0.010 0.167
Gly 990.96 1305.97 <0.001 1106.07 1190.86 <0.001 1070.43 1226.51 <0.001 <0.001 <0.001
Thr 1110.73 1415.99 <0.001 1317.49 1209.23 0.007 1187.90 1338.83 0.001 0.487 0.058
Arg 147.42 67.59 <0.001 58.74 156.26 <0.001 147.84 67.16 <0.001 <0.001 <0.001
Ala 3755.60 5075.63 <0.001 4702.66 4128.57 <0.001 4200.47 4630.76 <0.001 0.008 <0.001
Tau 9900.95 10535.96 0.05 10943.19 9493.73 <0.001 9995.35 10441.57 0.027 0.053 0.059
GABA 243.83 453.24 <0.001 329.71 367.37 0.100 489.56 207.52 <0.001 0.020 0.385
Tyr 348.52 195.85 <0.001 362.87 181.50 <0.001 168.10 376.27 <0.001 0.560 <0.001
Val 1528.90 1952.47 <0.001 1797.72 1683.64 0.003 1645.84 1835.53 <0.001 0.007 0.005
Met 960.55 1065.74 <0.001 1035.74 990.55 0.047 980.54 1045.75 0.010 0.248 0.259
Try 202.24 236.26 0.034 305.65 132.85 <0.001 249.80 188.71 0.002 0.003 0.007
Phe 1406.49 1422.54 0.680 1335.25 1493.77 0.003 1442.50 1386.52 0.174 0.520 0.021
Iso 1186.17 1414.23 <0.001 1319.51 1280.90 0.198 1282.39 1318.01 0.990 0.011 0.314
Leu 2222.27 2424.62 0.005 2321.24 2325.64 0.934 2323.77 2323.12 <0.001 0.084 0.017
Lys 998.14 843.28 <0.001 987.36 854.07 0.001 802.87 1038.56 <0.001 0.171 <0.001
Pro 547.15 1185.28 <0.001 1091.34 641.09 <0.001 707.76 1024.67 <0.001 <0.001 0.002
TFA 32058.79 37621.36 <0.001 35598.66 34081.49 0.018 32647.88 37032.28 <0.001 0.060 0.962