Table 3. Free amino acids contents of top round and shank muscles with three factors (muscle type, aging method, and aging time)
mg/kg | Muscle type | | Aging methods | | Aging time (d) | | p-value |
|
Top round | Shank | p-value | Dry | Wet | p-value | 20 | 40 | p-value | MT*AM1) | AM*AT2) |
Asp | 225.67 | 793.67 | <0.001 | 527.49 | 491.85 | <0.001 | 263.54 | 755.80 | <0.001 | <0.001 | <0.001 |
Glu | 2462.12 | 4247.87 | <0.001 | 3574.64 | 3135.34 | <0.001 | 2938.23 | 3771.76 | <0.001 | <0.001 | <0.001 |
Asn | 410.80 | 175.44 | <0.001 | 112.73 | 473.50 | <0.001 | 249.74 | 336.49 | <0.001 | <0.001 | <0.001 |
Ser | 1109.44 | 1020.65 | 0.014 | 1050.42 | 1079.67 | 0.332 | 1132.90 | 997.19 | 0.001 | 0.005 | 0.011 |
Gln | 1629.52 | 771.27 | <0.001 | 330.84 | 2069.94 | <0.001 | 576.62 | 1824.17 | <0.001 | 0.549 | <0.001 |
His | 671.32 | 1017.81 | <0.001 | 1023.63 | 665.50 | <0.001 | 791.75 | 897.38 | 0.021 | 0.010 | 0.167 |
Gly | 990.96 | 1305.97 | <0.001 | 1106.07 | 1190.86 | <0.001 | 1070.43 | 1226.51 | <0.001 | <0.001 | <0.001 |
Thr | 1110.73 | 1415.99 | <0.001 | 1317.49 | 1209.23 | 0.007 | 1187.90 | 1338.83 | 0.001 | 0.487 | 0.058 |
Arg | 147.42 | 67.59 | <0.001 | 58.74 | 156.26 | <0.001 | 147.84 | 67.16 | <0.001 | <0.001 | <0.001 |
Ala | 3755.60 | 5075.63 | <0.001 | 4702.66 | 4128.57 | <0.001 | 4200.47 | 4630.76 | <0.001 | 0.008 | <0.001 |
Tau | 9900.95 | 10535.96 | 0.05 | 10943.19 | 9493.73 | <0.001 | 9995.35 | 10441.57 | 0.027 | 0.053 | 0.059 |
GABA | 243.83 | 453.24 | <0.001 | 329.71 | 367.37 | 0.100 | 489.56 | 207.52 | <0.001 | 0.020 | 0.385 |
Tyr | 348.52 | 195.85 | <0.001 | 362.87 | 181.50 | <0.001 | 168.10 | 376.27 | <0.001 | 0.560 | <0.001 |
Val | 1528.90 | 1952.47 | <0.001 | 1797.72 | 1683.64 | 0.003 | 1645.84 | 1835.53 | <0.001 | 0.007 | 0.005 |
Met | 960.55 | 1065.74 | <0.001 | 1035.74 | 990.55 | 0.047 | 980.54 | 1045.75 | 0.010 | 0.248 | 0.259 |
Try | 202.24 | 236.26 | 0.034 | 305.65 | 132.85 | <0.001 | 249.80 | 188.71 | 0.002 | 0.003 | 0.007 |
Phe | 1406.49 | 1422.54 | 0.680 | 1335.25 | 1493.77 | 0.003 | 1442.50 | 1386.52 | 0.174 | 0.520 | 0.021 |
Iso | 1186.17 | 1414.23 | <0.001 | 1319.51 | 1280.90 | 0.198 | 1282.39 | 1318.01 | 0.990 | 0.011 | 0.314 |
Leu | 2222.27 | 2424.62 | 0.005 | 2321.24 | 2325.64 | 0.934 | 2323.77 | 2323.12 | <0.001 | 0.084 | 0.017 |
Lys | 998.14 | 843.28 | <0.001 | 987.36 | 854.07 | 0.001 | 802.87 | 1038.56 | <0.001 | 0.171 | <0.001 |
Pro | 547.15 | 1185.28 | <0.001 | 1091.34 | 641.09 | <0.001 | 707.76 | 1024.67 | <0.001 | <0.001 | 0.002 |
TFA | 32058.79 | 37621.36 | <0.001 | 35598.66 | 34081.49 | 0.018 | 32647.88 | 37032.28 | <0.001 | 0.060 | 0.962 |