Table 1. Analytical condition of HPLC

Conditions
Model	LC-10Avp Shimadzu Co., JAPAN
Column	Shim-pack CLC-ODS (M), 25 cm
Mobile phase	Acetonitrile : 0.5% formic acid; 5 min, 85% B; 20 min, 80% B; 30 min, 20% B; 31 min, 85% B; 45 min, 85% B
Flow rate	0.8 mL/min
Inj. Volume	20 μL
Detection	UV-VIS Detector (280 nm)