Table 1. IC50 values of ACE-inhibitory activity in whey protein 35% hydrolysates of enzymes at different hydrolysis time

Enzyme		Hydrolysis time (h)
Enzyme		0	2	3	4
Alcalase	IC₅₀¹	0.972 ± 0.001^a	0.107 ± 0.003^a	0.126 ± 0.032^bc	0.317 ± 0.029^b
Chymotrypsin	IC₅₀	0.972 ± 0.001^a	0.180 ± 0.005^c	0.157 ± 0.004^b	0.121 ± 0.001^a
Pepsin	IC₅₀	0.972 ± 0.001^a	0.179 ± 0.012^b	0.177 ± 0.006^a	0.186 ± 0.008^ba
Protease M	IC₅₀	0.972 ± 0.001^a	0.153 ± 0.005^a	0.136 ± 0.002^a	0.154 ± 0.009^b
Protease S	IC₅₀	0.972 ± 0.001^a	0.108 ± 0.003^b	0.103 ± 0.010^ba	0.099 ± 0.006^ba
Trypsin	IC₅₀	0.972 ± 0.001^a	0.258 ± 0.012^b	0.196 ± 0.009^a	0.243 ± 0.010^b