| HPLC | Shiseido performance liquid chromatography | |
| Mobile Phase | A: 10 mM Ammonium Formate | A: 0.1% Formic acid in D.W |
| B: Acetonitrile | B: 0.1% Formic acid in Acetonitrile |
| Gradient | 60% (A: 0-9 min) → 60-50% (A: 9-12 min) → 50-20% (A: 12-16 min) → 20-60% (A: 16-20 min) |
| Flow rate | 0.3 mL/min |
| Column | Cadenza 5CD-C18(2.1 mm I.D. × 150 mm, 5 μm) |
|
| Mass Spectrometry | Thermo Finigan TSQ Quantum Discovery Max |
| Ion mode | ESI (Positive) |
| Capillary temp | 350℃ |
| Collision gas | Ar |
| Collision Energy | |
|
| Energy Precursor ion(m/z) | Fragment ion(m/z) |
|
| Meloxicam | 352 | 115,141 |
| Flunixin | 297 | 279,264 |
| Flunixin D3 | 300 | 282,264 |
| Tulathromycin | 577 | 116,158 |
| Azythromycin | 749 | 83,158 |
|