Table 4. Methionine-oxidized peptides of myosin isoform identified from three major porcine muscles by LC-MS/MS

Sequences Musclea) Observed Mr (expt) Mr (calc) Ion scoreb)
IEDEQALAMQLQK SM 759.86 1517.70 1516.72 47
Myosin-1 (1098-1110)c) 767.79 1533.57 1532.71 50
Myosin-4 (1096-1108)
LAQESTMDIENDKQQLDEK SM 746.40 2236.18 2235.38 55
Myosin-1 (1065-1083) SM 751.80 2252.37 2251.38 61
Myosin-4 (1063-1081) LT 751.73 2252.15 2251.38 53
PM 751.64 2251.90 2251.38 59
MQGTLEDQIISANPLLEAFGNAK SM 821.47 2461.39 2460.76 40
Myosin-1 (215-237) SM 826.71 2477.12 2476.76 48
Myosin-2 (215-237) LT 826.65 2476.91 2476.76 56
Myosin-4 (215-237)
MFLWMVTR SM 542.94 1083.87 1083.37 40
Myosin-1 (438-445) 551.16 1100.30 1099.37 45
Myosin-2 (438-445)
Myosin-4 (438-445)
SAETEKEMANMKEEFEK SM 678.28 2031.81 2031.22 59
Myosin-1 (846-862) 689.06 2064.15 2063.22 55
Myosin-4 (844-860)