Inhibitory effect of genomic DNA extracted from Pediococcus acidilactici on Porphyromonas gingivalis lipopolysaccharide-induced inflammatory responses

Young Hyeon Choi1, Bong Sun Kim1, Seok-Seong Kang1,*
Author Information & Copyright
1Department of Food Science and Biotechnology, Dongguk University, Goyang 10326, Korea.
*Corresponding Author: Seok-Seong Kang, E-mail:

© Copyright 2022 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 21, 2022 ; Revised: Oct 01, 2022 ; Accepted: Oct 06, 2022

Published Online: Oct 12, 2022


This study aimed to assess whether genomic DNA (gDNA) extracted from Pediococcus acidilactici inhibits Porphyromonas gingivalis lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 cells. Pretreatment with gDNA of P. acidilactici K10 or P. acidilactici HW01 for 15 h effectively inhibited P. gingivalis LPS-induced mRNA expression of interleukin (IL)-1b, IL-6, and monocyte chemoattractant protein (MCP)-1. Although both gDNAs did not dose-dependently inhibit P. gingivalis LPS-induced mRNA expression of IL-6 and MCP-1, they inhibited IL-1b mRNA expression in a dose-dependent manner. Moreover, pretreatment with both gDNAs inhibited the secretion of IL-1b, IL-6, and MCP-1. When RAW 264.7 cells were stimulated with P. gingivalis LPS alone, the phosphorylation of mitogen-activated protein kinases (MAPKs) was increased. However, the phosphorylation of MAPKs was reduced in the presence of gDNAs. Furthermore, both gDNAs restored IkBa degradation induced by P. gingivalis LPS, indicating that both gDNAs suppressed the activation of nuclear factor-kB (NF-kB). In summary, P. acidilactici gDNA could inhibit P. gingivalis LPS-induced inflammatory responses through the suppression of MAPKs and NF-kB, suggesting that P. acidilactici gDNA could be effective in preventing periodontitis.

Keywords: Pediococcus acidilactici; genomic DNA; Porphyromonas gingivalis; Periodontitis; Inflammation

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