Article

The calcium solubilization ability and anti-inflammatory effect of hydrolyzed casein protein

Da-Young Kim1, Ho Sik Yoon2, Jung Sik Yoo1, Yoon Ah Cho1, Cheol-Hyun Kim1,*
Author Information & Copyright
1Dept. of Animal Resources Science, Dankook University, Cheonan-si 31116, Korea.
2Wellrunbnf Co., Cheonan-si 31188, Korea.
*Corresponding Author: Cheol-Hyun Kim, Dankook University, Cheonan-si 31116, Korea. Phone: 041-550-3656. E-mail: hichkim@dankook.ac.kr.

© Copyright 2021 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 12, 2020 ; Revised: Feb 22, 2021 ; Accepted: Jun 03, 2021

Published Online: Jun 04, 2021

Abstract

This study performed to evaluate the applicability of functional dairy food materials by comparing the calcium solubilization ability and anti-inflammatory effects of hydrolyzed casein protein. Commercial enzyme was added to the 10% casein solution to prepare the casein hydrolysates. Samples obtained every hour (1:200(w/v)). According to results of measuring the degree of hydrolysis (DH), all of four enzymatic hydrolysates increased rapidly from 30 to 40 minutes, and after 150 minutes, there were no change. Protamex® and Neutrase® had the highest DH compared to others enzymatic hydrolysates. After that, peptides obtained throughout a preparative liquid chromatography system. In the calcium solubility experiments, Neutrase Fraction(NF)4 and NF7 showed similar activities with CPP(casein phosphopeptide). In vitro cell experiments showed that no cytotoxicity except for NF6. Also, the production of nitric oxide (NO) inhibited as the concentration of fraction samples increased. The cytokine (IL-1α, IL-6, TNF-α) production was lower than lipopolysaccharide (+) group significantly. Therefore, the possibility of anti-inflammatory activity found in the hydrolyzed samples. According to the above experiments, NF3 and Protamex Fraction(PF)3 selected. Amino acids selected throughout an AccQ-Tag system. As a result, 17 species of amino acids and several species of unknown amino acids identified. Both fractions had the highest content of Phenylalanine. This study identified the potential of biologically active and functional peptides derived from casein that affect the food and dairy industry.

Keywords: Milk protein; Casein; calcium solubilization ability; Anti-inflammatory effect; Amino acid