Subacute Oral Toxicity Evaluation of Freeze-dried Powder of Locusta migratoria

Kyu-Won Kwak1,, Sun Young Kim1,, Kyu Sup An2, Yong-Soon Kim1, Kwanho Park1, Eunsun Kim1, Jae Sam Hwang1, Mi-Ae Kim1, Hyeon Yeol Ryu2,*, Hyung Joo Yoon1,*
Author Information & Copyright
1Department of Agricultural Biology, National Institute of Agricultural Science, Wanju, Jeollabuk-do 55365, Korea.
2Bioconvergence Technology Lab., Korea Conformity Laboratories, Incheon 21999, Korea.

† These authors contributed equally to this work.

*Corresponding Author: Hyeon Yeol Ryu, Bioconvergence Technology Laboratory, Korea Conformity Laboratories, Incheon 21999, Korea. E-mail:
*Corresponding Author: Hyung Joo Yoon, Department of Agricultural Biology, National Institute of Agricultural Science, Wanju 55365, Korea. E-mail:

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 30, 2020 ; Revised: Jul 15, 2020 ; Accepted: Jul 19, 2020

Published Online: Jul 23, 2020


Novel food sources have enormous potential as nutritional supplements. For instance, edible insects are considered as an alternative food source due to their higher protein content; moreover, they are economically efficient reproducers and have high in nutritional value. In this study, we investigated the toxicity of the freeze-dried powder of Locusta migratoria (fdLM), known to contain rich proteins as well as fatty acids. The objective of the present study was to evaluate the subacute toxicity of fdLM in male and female Sprague-Dawley (SD) rats. The SD rats were divided into four groups based on the dosage of fdLM administered: dosage of 0 (vehicle control), 750, 1500, and 3000 mg/kg/day were administered for 28 days. Toxicological assessments including observations on food consumption, body and organ weights, clinical signs, mortality, ophthalmologic tests, urinalyses, hematologic tests, clinical chemistry tests, gross findings, and histopathology tests were performed. Clinical signs, urinalyses, hematology, serum biochemistry tests, and organ weight examinations revealed no fdLM-related toxicity. The no-observed-adverse-effect level for fdLM was higher than 3000 mg/kg/day in rats of both sexes; therefore, fdLM, in conclusion, can be considered safe as an edible alternative human and animal food source material.

Keywords: Subacute toxicity; Locusta migratoria; freeze-dried powder; edible insects; Sprague-Dawley rats

Journal Title Change

We announce that the title of our journal and related information were changed as below from January, 2019.


Before (~2018.12)

After (2019.01~)

Journal Title

Korean Journal for Food Science of Animal Resources

Food Science of Animal Resources

Journal Abbreviation

Korean J. Food Sci. An.

Food Sci. Anim. Resour.







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