Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice
Received: Aug 19, 2019 ; Revised: Dec 13, 2019 ; Accepted: Dec 24, 2019
Published Online: Jan 17, 2020
The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 2025 g were randomly assigned to seven groups: CON, control; SCO, scopolamine (1 mg/kg body weight (B.W.), intraperitoneal (i.p.)); THA 10, tacrin (10 mg/kg B.W., per oral (p.o.)); PSW 10 (10 mg/kg B.W., p.o.); PSW 40, (40 mg/kg B.W., p.o.); LPSW 100 (100 mg/kg B.W., p.o.), and LPSW 400, (400 mg/kg B.W., p.o.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 µM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly effect on physical performance of mice. LPSW at 400 mg/kg significantly increased spontaneous alternation, reaching the level observed for THA (10 mg/kg B.W.) and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine content and decreased acetylcholine esterase activity (p < 0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.