Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice

Dongwook Kim1, Yuan H. Brad Kim2, Jun-Sang Ham3, Sung Ki Lee1, Aera Jang1,*
Author Information & Copyright
1Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea
2Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA
3Animal Products and Utilization Division, National Institute of Animal Science, RDA, Korea
*Corresponding Author : Aera Jang. Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea. Tel: +82-33-250-8643, Fax: +82-33-251-7719, E-mail:

© Copyright 2020 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 19, 2019 ; Revised: Dec 13, 2019 ; Accepted: Dec 24, 2019

Published Online: Jan 17, 2020


The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 2025 g were randomly assigned to seven groups: CON, control; SCO, scopolamine (1 mg/kg body weight (B.W.), intraperitoneal (i.p.)); THA 10, tacrin (10 mg/kg B.W., per oral (p.o.)); PSW 10 (10 mg/kg B.W., p.o.); PSW 40, (40 mg/kg B.W., p.o.); LPSW 100 (100 mg/kg B.W., p.o.), and LPSW 400, (400 mg/kg B.W., p.o.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 µM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly effect on physical performance of mice. LPSW at 400 mg/kg significantly increased spontaneous alternation, reaching the level observed for THA (10 mg/kg B.W.) and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine content and decreased acetylcholine esterase activity (p < 0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

Keywords: hydrolysates; pig skin gelatin; cognitive; scopolamine; learning and memory

Journal Title Change

We announce that the title of our journal and related information were changed as below from January, 2019.


Before (~2018.12)

After (2019.01~)

Journal Title

Korean Journal for Food Science of Animal Resources

Food Science of Animal Resources

Journal Abbreviation

Korean J. Food Sci. An.

Food Sci. Anim. Resour.







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