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Comparative Genome Analysis and Evaluation of Probiotic Characteristics of Lactobacillus plantarum Strain JDFM LP11
Korean J. Food Sci. An. 2018;38:878-888
Published online October 31, 2018;  https://doi.org/10.5851/kosfa.2018.e21
© 2018 Korean Society for Food Science of Animal Resources

Jaeyoung Heo1,†, Donghyun Shin2,†, Sung Yong Chang3, Paul Bogere4, Mi Ri Park3, Sangdon Ryu3, Woong Ji Lee3, Bohyun Yun3, Hak Kyo Lee2, Younghoon Kim3, and Sangnam Oh5,*

1International Agricultural Development and Cooperation Center, Chonbuk National University, Jeonju 54896, Korea
2Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea
3Department of Animal Science and Institute of Milk Genomics, Chonbuk National University, Jeonju 54896, Korea
4Graduate School of Agricultural Convergence Technology, Chonbuk National University, Jeonju 54896, Korea
5Department of Functional Food and Biotechnology, Jeonju University, Jeonju 55069, Korea
Correspondence to: Sangnam Oh
Department of Functional Food and Biotechnology, Jeonju University, Jeonju 55069, Korea
Tel: +82-63-220-3109
Fax: +82-63-220-2054
E-mail: osangnam@jj.ac.kr
Received May 28, 2018; Revised July 16, 2018; Accepted July 24, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In the current study, the probiotic potential of approximately 250 strains of lactic acid bacteria (LAB) isolated from piglet fecal samples were investigated; among them Lactobacillus plantarum strain JDFM LP11, which possesses significant probiotic potential, with enhanced acid/bile tolerance, attachment to porcine intestinal epithelial cells (IPEC-J2), and antimicrobial activity. The genetic characteristics of strain JDFM LP11 were explored by performing whole genome sequencing (WGS) using a PacBio system. The circular draft genome have a total length of 3,206,883 bp and a total of 3,021 coding sequences were identified. Phylogenetically, three genes, possibly related to survival and metabolic activity in the porcine host, were identified. These genes encode p60, lichenan permease IIC component, and protein TsgA, which are a putative endopeptidase, a component of the phosphotransferase system (PTS), and a major facilitator in the gut environment, respectively. Our findings suggest that understanding the functional and genetic characteristics of L. plantarum strain JDFM LP11, with its candidate genes for gut health, could provide new opportunities and insights into applications in the animal food and feed additive industries.
Keywords : probiotics, L. plantarum, genome analysis, gut health


October 2018, 38 (5)