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Prevalence, Serotype Diversity, Genotype and Antibiotic Resistance of Listeria monocytogenes Isolated from Carcasses and Human in Korea
Korean J. Food Sci. An. 2018;38:851-865
Published online October 31, 2018;  https://doi.org/10.5851/kosfa.2018.e5
© 2018 Korean Society for Food Science of Animal Resources

Hyemin Oh1,2, Sejeong Kim1,2, Soomin Lee1,2, Heeyoung Lee1,2, Jimyeong Ha1,2,
Jeeyeon Lee1,2, Yukyung Choi1,2, Kyoung-Hee Choi3,*, and Yohan Yoon1,2,*

1Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
2Risk Analysis Research Center, Sookmyung Women’s University, Seoul 04310, Korea
3Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan 54538, Korea
Correspondence to: Yohan Yoon
Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Tel: +82-2-2077-7585
Fax: +82-2-710-9479
E-mail: yyoon@sookmyung.ac.kr

Kyoung-Hee Choi
Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan 54538, Korea
Tel: +82-63-850-6911
Fax: +82-63-850-6911
E-mail: kheechoi@wku.ac.kr
Received February 19, 2018; Revised May 29, 2018; Accepted June 3, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study investigated the prevalence of Listeria monocytogenes in slaughterhouses, and determined serovars and genotypes, and antibiotic resistance of the isolates obtained from slaughterhouses and humans in Korea. Two hundred ninety samples were collected from feces (n=136), carcasses [n=140 (cattle: n=61, swine: n=79)], and washing water (n=14) in nine slaughterhouses. Eleven human isolates were obtained from hospitals and the Korea Center for Disease Control and Prevention. Listeria monocytogenes was enriched and identified, using polymerase chain reaction (PCR) and 16S rRNA sequencing. Serovars and presence of virulence genes were determined, and genetic correlations among the isolates were evaluated by the restriction digest patterns of AscI. Antibiotic resistance of L. monocytogenes isolates were examined against 12 different antibiotics. Of 290 slaughterhouse samples, 15 (5.17%) carcass samples were L. monocytogenes positive. Most L. monocytogenes isolates possessed all the virulence genes, while polymorphisms in the actA gene were found between carcass and human isolates. Serovars 1/2a (33.3%) and 1/2b (46.7%) were the most frequent in carcass isolates. Genetic correlations among the isolates from carcass and clinical isolates were grouped within serotypes, but there were low geographical correlations. Most L. monocytogenes isolates were antibiotic resistant, and some strains showed resistance to more than four antibiotics. These results indicate that L. monocytogenes are isolated from carcass and human in Korea, and they showed high risk serotypes and antibiotic resistance. Therefore, intensive attentions are necessary to be aware for the risk of L. monocytogenes in Korea.
Keywords : Listeria monocytogenes, slaughterhouse, epidemiological analysis, molecular typing, antibiotic


October 2018, 38 (5)