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Investigation of the Protective Effect of Kefir against Isoproterenol Induced Myocardial Infarction in Rats
Korean J. Food Sci. An. 2018;38:259-272
Published online April 30, 2018
© 2018 Korean Society for Food Science of Animal Resources

Handan Mert1,*, Hikmet Yılmaz1, Kıvanç Irak2, Serkan Yıldırım3, and Nihat Mert1

1Department of Biochemistry, Yuzuncu Yil University, Faculty of Veterinary Medicine, Van 65090, Turkey
2Department of Biochemistry, Siirt University, Faculty of Veterinary Medicine, Siirt, 56100, Turkey
3Department of Pathology, Ataturk University, Faculty of Veterinary Medicine, Erzurum, 25030, Turkey
Correspondence to: Handan Mert
Department of Biochemistry, Yuzuncu Yil University, Faculty of Veterinary Medicine, Van 65090, Turkey
Tel: +904322251128 Fax: +904322251127 E-mail:
Received December 27, 2017; Revised January 26, 2018; Accepted January 26, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study aims to investigate the protective effects of kefir against myocardial infarction induced by isoproterenol (ISO). The rats were randomly divided into 4 groups, each group consisting of 8 rats. The control group, the kefir group (5 mL/kg/d kefir administered to rats as intra-gastric gavage for 60 d), the ISO group (100 mg/kg ISO was administered to rats, s.c. on 61. and 62. d), and kefir+ISO group (5 mL/kg/d kefir was administered to rats intra gastric gavage for 60 days prior to ISO, 100 mg/kg in two doses on day 61 and 62). 12 h after the last ISO dose, all rats were decapitated and their blood samples were collected. Cardiac tissue was reserved for histopathological examination. creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), triglycerides, total cholesterol,very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) and glucose were measured by autoanalyzer, whole blood malondialdehyde (MDA), glutathione (GSH) and plasma advanced oxidation protein products (AOPP) levels were measured spectrophotometrically. It was determined that in the group of kefir+ISO, the levels of AST (p<0.001), CK (p<0.001), LDH (p<0.001), MDA (p<0.001) and AOPP (p<0.001) were decreased, while the GSH (p<0.05) increased, compared to ISO group. There were no significant changes in lipid profile and glucose levels between these two groups. In conclusion, by examining cardiac enzymes and histopathological changes in cardiac tissue, it can be concluded that the administration of kefir in myocardial infarction induced by ISO can protect the heart with its antioxidant characteristic and minimize the toxic damage created by ISO.
Keywords : antioxidant, cardiac marker, isoproterenol, kefir, myocardial infarction

August 2018, 38 (4)