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Optimizing Medium Components for the Maximum Growth of Lactobacillus plantarum JNU 2116 Using Response Surface Methodology
Korean J. Food Sci. An. 2018;38:240-250
Published online April 30, 2018
© 2018 Korean Society for Food Science of Animal Resources

Heeseop Yoo1, Insoo Rheem2, Sungsue Rheem3,*, and Sejong Oh4,*

1JNBIO, Jeonnam 57509, Korea
2Department of Laboratory Medicine, Dankook University Hospital, Cheonan 31116, Korea
3Graduate School of Public Administration, Korea University, Sejong 30019, Korea
4Division of Animal Science, Chonnam National University, Gwangju 61186, Korea
Correspondence to: Sejong Oh
Division of Animal Science, Chonnam National University, Gwangju 61186, Korea
Tel: +82-62-530-2116 Fax: +82-62-530-2129 E-mail: soh@jnu.ac.kr
Sungsue Rheem
Graduate School of Public Administration, Korea University, Sejong 30019, Korea
Tel: +82-44-860-1554 Fax: +82-44-860-1551 E-mail: rheem@korea.ac.kr
Received January 24, 2018; Revised January 30, 2018; Accepted January 31, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study was undertaken to find the optimum soy-peptone, glucose, yeast extract, and magnesium sulfate amounts for the maximum growth of Lactobacillus plantarum JNU 2116 and to assess the effects of these medium factors through the use of response surface methodology. A central composite design was used as the experimental design for the allocation of treatment combinations. In the analysis of the experiment, due to a significant lack of fit of the second-order polynomial regression model that was used at first, cubic terms were added to the model, and then two-way interaction terms were deleted from the model since they were found to be all statistically insignificant. A relative comparison among the four factors showed that the growth of L. plantarum JNU 2116 was affected strongly by yeast extract, moderately by glucose and peptone, and slightly by magnesium sulfate. The estimated optimum amounts of the medium factors for the growth of L. plantarum JNU 2116 are as follows: soy-peptone 0.213%, glucose 1.232%, yeast extract 1.97%, and magnesium sulfate 0.08%. These results may contribute to the production of L. plantarum L67 as a starter culture that may have potential application in yogurt and fermented meat products.
Keywords : response surface methodology, optimization, growth, Lactobacillus plantarum


April 2018, 38 (2)