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Inhibition of Clostridium perfringens using Bacteriophages and Bacteriocin Producing Strains
Korean J. Food Sci. An. 2018;38:88-98
Published online February 28, 2018
© 2018 Korean Society for Food Science of Animal Resources

Sunhak Heo, Min Gon Kim, Mirae Kwon, Hee Soo Lee, and Geun-Bae Kim*

Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Correspondence to: Geun-Bae Kim
Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Anseong 17546, Korea
Tel: +82-31-670-3027
Fax: +82-31-676-5986
Received December 8, 2017; Revised December 21, 2017; Accepted December 21, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against Clostridium perfringens, from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as Streptococcus hyointestinalis by 16S rRNA gene sequencing. The bacteriocin from the isolated strain exhibited strong inhibitory activity against four strains of C. perfringens and all the tested strains of Listeria monocytogenes, and the bacteriocin were highly heat- and pH-stable even at pH 2, pH 10 and 121°C for 15 min. We also evaluated the combined effects of the isolated bacteriocin and phages. Combining the phage treatments and bacteriocin resulted in a synergetic effect compared with the phage or the bacteriocin alone. In addition, during the probiotic test, the bacteriocin-producing S. hyointestinalis B19 strain reduced the population of C. perfringens significantly. Treatment with S. hyointestinalis B19 and a cocktail of lytic bacteriophages eradicated the C. perfringens KCTC 3269T, completely. Consequently, the isolated bacteriocin and bacteriophages represent candidates for effective biocontrol of C. perfringens, and bacteriocin-producing S. hyointestinalis B19 is a potential probiotic candidate for use in domestic animals.
Keywords : bacteriocin, bacteriocin-producing bacteria, bacteriophage, Clostridium perfringens, Streptococcus hyointestinalis

August 2018, 38 (4)