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Whey Protein Attenuates Angiotensin II-Primed Premature Senescence of Vascular Smooth Muscle Cells through Upregulation of SIRT1
Korean J. Food Sci. An. 2017;37:917-925
Published online December 31, 2017
© 2017 Korean Society for Food Science of Animal Resources

Jung Seok Hwang, Sung Gu Han, Chi-Ho Lee, and Han Geuk Seo*

Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, Korea
Correspondence to: Han Geuk Seo
Department of Food Science and Biotechnology of Animal Products, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, Korea
Tel: +82-2-450-0428 Fax: +82-2-455-1044 E-mail: hgseo@konkuk.ac.kr
Received November 2, 2017; Revised November 28, 2017; Accepted November 28, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.
Keywords : angiotensin II, senescence, SIRT1, vascular smooth muscle cell, whey protein


December 2017, 37 (6)