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Effects of Fermented Milk with Mixed Strains as a Probiotic on the Inhibition of Loperamide-Induced Constipation
Korean J. Food Sci. An. 2017;37:906-916
Published online December 31, 2017
© 2017 Korean Society for Food Science of Animal Resources

Byoung-Kook Kim1, In Suk Choi1, Jihee Kim1, Sung Hee Han2*, Hyung Joo Suh3, and Jae-Kwan Hwang*

Department of Biotechnology & Department of Biomaterials Science and Engineering, Yonsei University, Seoul 03722, Korea
1Chong Kun Dang Bio Research Institute, Gyeonggi-do 15064, Korea
2BK21Plus, College of Health Science, Korea University, Seoul 02841, Korea
3Department of Public Health Sciences, Graduate School, Korea University, Seoul 02841, Korea
Correspondence to: Sung Hee Han
BK21Plus, College of Health Science, Korea University, Seoul 02841, Korea
Tel: +82-2-3290-5639 Fax: +82-2-921-7207 E-mail: sunghee3@gmail.com
Jae-Kwan Hwang
Department of Biotechnology & Department of Biomaterials Science and Engineering, Yonsei University, Seoul 03722, Korea
Tel: +82-2-2123-5881 Fax: +82-2-362-7265 E-mail: jkhwang@yonsei.ac.kr
Received October 12, 2017; Revised November 23, 2017; Accepted November 24, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To investigate the effects of a single bacterium and a mixture of bacteria as probiotics in loperamide-treated animal models, loperamide (3 mg/kg) was administered to SD rats to induce constipation. The individual lactic acid bacterial doses, Enterococcus faecium (EF), Lactobacillus acidophilus (LA), Streptococcus thermophilus (ST), Bifidobacterium bifidum (BB), Bifidobacterium lactis (BL), Pediococcus pentosaceus (PP), and a mixture of the bacteria were orally administered to loperamide-induced constipated rats at a concentration of 108 CFU/kg for 14 days. The weights and water contents of their stools were found to be significantly higher in PP, CKDB (mixture of 5 strains except PP), and CKDBP (CKDB+PP) groups than in the normal (constipation not induced) and the control (constipation-induced) groups (P<0.05). The intestinal transit ratio was significantly higher in all probiotic-treated groups than in the control group, and was the highest in the CKDBP group (P<0.05). The mucosal length and mucus secretion were significantly improved in all probiotic-treated-groups, as compared to that in the control group, and the CKDBP group was found to be the most effective according to immunohistochemistry (IHC) staining and total short chain fatty acid content analysis (P<0.05). Lastly, PP, CKDB, and CKDBP showed relatively higher Lactobacillus sp. ratios of 61.94%, 60.31% and 51.94%, respectively, compared to the other groups, based on metagenomic analysis.
Keywords : constipation, probiotic, bacteria, CKDBP, loperamide


December 2017, 37 (6)