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Evaluation on Antimicrobial Activity of Psoraleae semen Extract Controlling the Growth of Gram-Positive Bacteria
Korean J. Food Sci. An. 2017;37:502-510
Published online August 31, 2017
© 2017 Korean Society for Food Science of Animal Resources

Sejeong Kim1,2, Soomin Lee1,2, Heeyoung Lee1,2, Jimyeong Ha1,2, Jeeyeon Lee1,2, Yukyung Choi1,2, Hyemin Oh1,2, Joonbae Hong3, Yohan Yoon1,2*, and Kyoung-Hee Choi4,5*

1Department of Food and Nutrition, Sookmyung Women's University
2Risk Analysis Research Center, Sookmyung Women's University
3Test & Research Department, Consumer Safety Center, Korea Consumer Agency
4Department of Oral Microbiology, College of Dentistry, Wonkwang University
5Institute of Biomaterials-Implant, Wonkwang University
Correspondence to: Kyoung-Hee Choi
College of Dentistry, Wonkwang University, Iksan 54538, Korea
Tel: +82-63-850-6911 Fax: +82-63-850-6911 E-mail:
Yohan Yoon
Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea
Tel: +82-2-2077-7585 Fax: +82-2-710-9479 E-mail:
Received February 8, 2017; Revised June 21, 2017; Accepted July 4, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were 8 μg/mL for Streptococcus mutans, and 16 μg/mL for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration 8-16 μg/mL. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.
Keywords : antibacterial activity, natural antimicrobials, therapeutic herbal plants

August 2017, 37 (4)