Table 1. Primer sets of M1 or M2 macrophage signature genes analyzed by quantitative real-time reverse transcriptase-PCR (qRT-PCR)
Gene name | Classification | Primer sequence (5′→ 3′) | Tm (°C) | Product size | Accession number |
anxa1 | M2 macrophage marker | F: AGCTCTGGATCTGGAACTGA | 55.4 | 103 | NM_010730.2 |
R: TTCGTACAGCTTCTCGGCAA | 55.4 |
mafb | M2 macrophage marker | F: GCGAGCAACTACCAGCAGAT | 57.4 | 125 | NM_010658 |
R: GCACTACGGAAGCCGTCGAA | 59.5 |
sepp1 | M2 macrophage marker | F: GAGCATCTTGGCAGCAGTAA | 55.4 | 140 | NM_001042614 |
R: GTGGTGTCTCAGCTCTCTAA | 55.4 |
hdac9 | M1 macrophage marker | F: ATCAGCTCAGTGGACGTGAA | 55.4 | 103 | NM_024124 |
R: GATCCACCACAGGCATCATC | 57.4 |
ptgs2 | M1 macrophage marker | F: ATCAGGTCATTGGTGGAGAGG | 58.2 | 137 | NM_011198 |
R: CATCAGACCAGGCACCAGA | 57.3 |
socs3 | M1 macrophage marker | F: CCTCAAGACCTTCAGCTCCAA | 58.2 | 82 | NM_007707 |
R: CCAGTAGAATCCGCTCTCCT | 57.4 |