| Bovine | Chondrocytes | Chemically defined medium (CDM) | Transforming growth factor (TGF)-β3 | The treatment group to which TGF-β3 was added without the addition of FBS showed excellent cell growth. | Byers et al. (2008) |
| Dulbecco’s modified Eagle’s medium (DMEM) | - | The spontaneous calcium signaling in chondrocytes is affected by FBS exposure. | Zhou et al. (2015) |
| High glucose DMEM | - | Cartilage tissue was formed through a three-dimensional culture system in a medium without serum and growth factors. | Ahmed et al. (2014) |
| Corneal endothelium cells | DMEM/F12 | Platelet releasates | Human platelet releasates used for ex vivo expansion of corneal endothelial cells. | Chou et al. (2014) |
| Cumulus-oocyte complexes | Modified synthetic oviduct fluid (mSOF) | Insulin, transferrin, and selenium (ITS), bovine serum albumin (BSA) | Investigation of the necessity and possible interactions of serum, cumulus cells, and oil during cell culture. | Goovaerts et al. (2012) |
| Follicle | McCoy’s 5a medium | Insulin-like growth factor (IGF)-I (hrIGF-I) | Investigation of culture and development of follicles through regulation of IGF-I bioavailability. | Thomas et al. (2007) |
| Granulosa cells | Modified alpha-minimum essential medium (MEM α) | 0.1% polyvinyl alcohol | Stimulation of progesterone production in granulosa cells through the addition of norepinephrine. | Piccinato et al. (2012) |
| Keratinocytes (Comea) | DMEM/F12 | | IGF-II and collagen expression in keratocytes. | Kane et al. (2009) |
| Nucleus pulposus cells | CDM | TGF-β3 | Comparison of cell development in serum-free/serum-supplemented media containing TGF-β3. | Reza and Nicoll (2010) |
| Oocyte | KSOM–PVA (potassium-supplemented simplex optimized medium-polyvinyl-alcohol) | Glucose, fructose | Effect of the addition of glucose and fructose to the supplemented serum-free medium on oocyte development. | Bhuiyan et al. (2007) |
| McCoy’s 5a | Activin, follicle-stimulating hormone | Identification of ingredients that help the growth and activation of oocytes. | McLaughlin and Telfer (2010) |
| Primary bovine satellite cells (BSCs) | Beefy-9 | - | Confirmation of the effect of reducing the cost of cultured meat production by developing a serum-free medium. | Stout et al. (2022) |
| Primary bovine myoblasts | Seven commercial serum-free media | LipoGroTM, XerumFreeTM | Comparison of cell growth effects of 7 commercial serum-free media and 3 additives. | Kolkmann et al. (2020) |
| Umbilical cord Wharton’s jelly cells | Stemline® mesenchymal stem cell expansion medium | - | Comparison of cell growth and morphology in commercial serum-free medium. | Cardoso et al. (2012) |
| Whole embryo | HEPES buffered TCM 199 medium | Growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF), recombinant albumin (RA), hyaluronan (HA) | Development of fetal calf serum or BSA free medium using various growth factors and cytokines or other molecules with surfactant and embryotrophic properties. | Moreno et al. (2015) |
| Two-step defined culture system (C1/C2 medium) | - | Development of a defined culture medium that supported improved in vitro bovine embryo development and calving rate after embryo transfer. | Lim et al. (2007) |
| - | Evaluation of the developmental competence of bovine embryos in the serum-free medium at early or later embryonic stages. | Park et al. (2010) |
| mSOF | CDM, BSA | Comparison of blastocyst development in embryos cultured in serum-supplemented and serum-free media. | Jang et al. (2011) |
| Dulbecco’s phosphate buffered saline-sericin (cryopreservation) | Silk protein sericin | Effect of silk protein sericin in serum-free freezing of bovine embryos. | Isobe et al. (2013) |
| TCM-199 medium | Albumin, ITS | Evaluation of the development of bovine embryos and blastocyst quality in serum-free conditions. | Wydooghe et al. (2014) |
| Porcine | Bone marrow-derived mesenchymal stromal cells | Ultra-CULTURE | Epithelial growth factor (EGF) | Confirmation of substitutability of the serum-free medium through the addition of growth factors. | Wang et al. (2013) |
| Chondrocytes | Ham F12 | Autologous serum, fibroblast growth factor—basic (FGF-basic), IGF-1 | Indentification of serum replacement effect of autologous serum and growth factors in serum-free medium. | Kamil et al. (2007) |
| Hepatocytes | DMEM/199 medium | Dimethyl sulfoxide (DMSO), dexamethasone | Verification of cell monolayer formation and maintenance in serum-free medium condition. | Caperna et al. (2011) |
| Williams E medium | - | Comparison of phenotype-induced changes in cells cultured in serum-free/serum-supplemented media. | Rasmussen (2017) |
| Oocyte | Modified porcine oocyte medium (mPOM) | - | Defined system for in vitro production of porcine embryos. | Yoshioka et al. (2008) |
| In vitro production studies of pig embryos in a defined serum-free medium. | Akaki et al. (2009) |
| - | In vitro-derived blastocyst quality improvement study in a defined serum-free medium. | Koike et al. (2010) |
| - | Study of interaction between oocytes during in vitro maturation in a defined serum-free medium. | Appeltant et al. (2015) |
| Modified medium 199 | - | Developmental capacity of selected oocytes to mature in a defined serum-free medium. | Ishizaki et al. (2009) |
| North Carolina State University-23 (NCSU-23) Modified NCSU-23 medium | - | Effect of amino acid addition on maturation, fertilization, and preimplantation development of oocytes. | Hong and Lee (2007) |
| Whole embryo | Porcine zygote medium (PZM)-5 | | Defined system for in vitro production of porcine embryos. | Yoshioka et al. (2008) |
| PZM-4 | | Subsequent embryo development in a defined serum-free medium using selected oocytes. | Ishizaki et al. (2009) |
| PZM-3/4/5 | | Effects of the CDM on the early development of porcine embryos. | Cao et al. (2012) |
| Glucose, glycine | The effects of glucose and/or glycine on the in vitro development of porcine blastocysts. | Mito et al. (2012) |
| Porcine blastocyst medium, PZM-5 | | Birth of piglets from in vitro-produced porcine blastocysts vitrified and warmed in a chemically defined medium. | Mito et al. (2015) |
| TCM-199–HEPES (TCM) | Polyvinyl alcohol | Vitrification and warming of in vivo-derived porcine embryos in a chemically defined medium. | Sanchez-Osorio et al. (2010) |
| Chicken | PBS-12SF (original CHCC-OU2 line) | OptiPROTM SFM | - | Evaluation of immortalized chick embryo cell lines for vaccine production in serum-free conditions. | Coussens et al. (2011) |
| Duck | AGE1.CR, AGE1. CR.pIX | SFM-G, HyQ®SFM4MegaVirTM | - | Evaluation of the growth of avian designer cell lines in serum-free media for use in vaccine manufacturing. | Lohr et al. (2009) |
| Sheep | Oocyte | TCM 199 | Sericin, polyvinyl alcohol | Confirmation of FBS replacement effect of sericin. | Hajarian et al. (2017) |
| HEPES buffered TCM 199 | Growth factor, hormones | Comparison of oocyte development in serum-free medium supplemented with growth factors and hormones. | Arunakumari et al. (2010) |
| Goat | Whole embryo | mSOF, DI / II | Bovine serum albumin, polyvinyl alcohol | Identification of optimal medium conditions and cryotolerance levels for culturing goat embryos. | Choi et al. (2016) |