Table 3 Amount of protein in the CFS obtained from E. faecalis KT11 and bacteriocin activity of CFS against S. aureus ATCC 25923 at various purification stages

Purification stages	Volume(mL)	Protein(mg/mL)	Total proteina(mg)	Activityb(AU/mL)	Total activityc(AU)	Specific activityd(AU/mg)	Purificationfactore	Recoveryf(%)
CFS	50.0	0.3286	16.4300	178	8,900	541.69	1.0	100
Ammonium sulfate precipitation	3.0	0.0507	0.1520	711	2,133	14,014.45	25.87	23.96
Dialysis	2.0	0.0498	0.09976	711	1,422	14,254.21	26.31	15.97

^a Total protein: Amount of protein determined by the Bradford method×total volume (mL).
^b Activity units (AU/mL): Reciprocal of the highest dilution×1,000/volume of bacteriocin added.
^c Total activity (AU): Activity unit×total volume (mL).
^d Specific activity (AU/mg): Total activity of the subsequent purification step/total protein of the same step.
^e Purificaton factor: Specific activity of subsequent purification step/specific activity of the crude preparation.
^f Recovery (%): Total activity of the subsequent purification step/total activity of the crude preparation×100.